哺乳期乳房脓肿患者脓液涂片镜检与培养结果的一致性分析及主要致病菌的药敏结果

Consistency analysis of pus smear microscopy and culture results in breast abscess patients during lactation and drug susceptibility of major pathogenic bacteria

  • 摘要:
      目的  探讨哺乳期乳房脓肿患者脓液涂片革兰染色镜检与最终培养结果的一致性,分析主要致病菌金黄色葡萄球菌的药敏结果。
      方法  对123位哺乳期乳房脓肿患者的脓液标本进行革兰染色镜检和常规需氧培养,并对培养出的89例金黄色葡萄球菌进行药敏试验。
      结果  123例脓液标本涂片检出细菌103例,与培养结果一致率为92.2%; 涂片未检出细菌20例,与培养结果一致率为55.0%。培养出金黄色葡萄球菌89株,占培养阳性例数84.0%,且各年份间金黄色葡萄球菌占培养阳性的比例及对常用抗生素的耐药率差异无统计学意义(P>0.05)。
      结论  对哺乳期乳房脓肿患者的脓液标本进行涂片革兰染色镜检结果与培养结果的一致性较高,且分离的金黄色葡萄球菌对常用抗生素的耐药率稳定,本研究可以给临床医师经验性用药提供依据促进抗生素的合理使用,协助临床快速诊断。

     

    Abstract:
      Objective  To investigate the consistency of Gram staining microscopy and final culture results of abscess smears from breast abscess patients in lactation period, and to analyze the drug susceptibility results of staphylococcus aureus.
      Methods  Totally 123 breast abscess specimens from breast-feeding patients were examined by Gram staining microscopy and conventional aerobic culture, and 89 staphylococcus aureus specimens were cultured for drug susceptibility test.
      Results  Totally 103 bacteria were detected in 123 pus specimens, the coincidence rate with culture results was 92.2%. Totally 20 bacteria were not detected in the smear, and the coincidence rate with culture results was 55.0%. Totally 89 strains of staphylococcus aureus were cultured, which accounted for 84.0% of the positive cases. There was no significant difference in the proportion of staphylococcus aureus in the positive cases and the resistance rate to commonly used antibiotics between years (P>0.05).
      Conclusion  Gram staining microscopic examination of breast abscess specimens from breast-feeding patients is highly consistent with culture results, and the drug resistance rate of isolated staphylococcus aureus to commonly used antibiotics is stable, which can provide evidence for clinicians to use empirical drugs, promoto the rational use of antibiotics and assist the rapid clinical diagnosis.

     

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