Objective  To explore the effect of acupoint application combined with clomiphene on pregnancy rate, levels of serum anti-mullerian hormone (AMH) and monocyte chemotactic protein-1 (MCP-1) in sterility patients with polycystic ovary syndrome (PCOS) differentiated as kidney deficiency and blood stasis.

  Methods  A total of 130 PCOS patients with sterility differentiated as kidney deficiency and blood stasis were selected, and were randomly divided into experimental group and control group, with 65 cases in each group. The control group was treated with clomiphene, and the experimental group was treated with acupoint application on the basis of the control group. Both groups were treated for 3 months. The number of ovarian sinus vesicles, scores of hirsutism and acne symptoms, serum sex hormones levelsfollicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), AMH and MCP-1 levels before and 3 months after treatment were detected. The pregnancy rates of the two groups were recorded after follow up for 12 months.

  Results  After 3 months of treatment, the number of bilateral ovarian sinus vesicles in two groups decreased, and the experimental group was less than that of control group (P < 0.05); after 3 months of treatment, the scores of hairy and acne symptoms, levels of FSH, LH, AMH, MCP-1 and T in two groups were lower than before treatment, and the above indicators in the experimental group were lower than that in the control group (P < 0.05). The pregnancy rates in the experimental group were higher than those in the control group at 9 and 12 months after treatment (P < 0.05).

  Conclusion  Acupoint application combined with clomiphene can alleviate clinical symptoms, improve sex hormone levels, reduce serum AMH, MCP-1 expression and improve prognosis pregnancy rate in sterility patients with PCOS differentiated as kidney deficiency and blood stasis.