Abstract: Objective To analyze the status and distribution of skin squamous cell carcinoma patients with human papilloma virus (HPV) infection and to explore the relationship between HPV infection and skin squamous cell carcinoma.Methods The PCR technique was used to detect HPV DNA in 290 patients with squamous cell carcinoma of the skin tissue samples after histopathological diagnosis,and the main detector type were 6,16 and 33.Results Among 290 cases with skin squamous cell carcinoma,196 cases had positive HPV,and HPV infection rate was 63.23％.HPV infection rate in male patients was 68.37％,and the rate was 31.63％ in female patients (P ＞ 0.05).Infection rate in high differentiation group (I ～II) was 67.87％,which was significantly higher than 47.38％ in low differentiation group (Ⅲ ～ IV) (P ＜0.05).Infection rates of HPV infection in regions such as head,face,neck,arms and legs,torso,and genitals,groin were 47.96％,26.53％,7.14％,26.53％,14.29％,and there were significant differences in different parts (P ＜ 0.05).Totally 57 cases were detected with high-risk type (HPV 16,33) infection,and 53 cases were with low-risk type (HPV6) infection.Conclusion HPV infection is significantly associated with squamous cell carcinoma of the skin.