Abstract: Objective To observe and analyze the application value of serum unconjugated bilirubin (UCB) and albumin (ALB) detections in children with neonatal jaundice.Methods A total of 285 children with neonatal jaundice were selected.The serum UCB,total bilirubin (TBIL),ALB and β2 microglobulin (β2-MG) levels of the children were detected and compared.Results The serum UCB,TBIL,UCB/ALB,β2-MG levels in children with severe jaundice were significantly higher than those with moderate jaundice (P ＜ 0.05).The serum UCB,TBIL,UCB/ALB,β2-MG levels in children with nuclear jaundice were significantly higher than those without nuclear jaundice (P ＜ 0.05).The serum UCB,TBIL,UCB/ALB,β2-MG levels in children with infectious jaundice were significantly higher than those with other three etiological types (P ＜ 0.05).Conclusion The serum UCB and UCB/ALB levels in the children with neonatal jaundice are correlated with the severity of the disease and the etiological type.