Abstract: Objective To explore the value of HPV-DNA combined with HPV-E6 protein in the detection of cervical cancer.Methods A total of 525 patients with positive TCT were selected and treated with HPV-DNA detection,HPV detection of E6 protein and cervical biopsy.Value of HPV-DNA and HPV-E6 proteins single application and combined detection for cervical cancer screening was compared.Results In the patients with positive TCT,375 cases were normal or cervicitis,134 cases were intraepithelial neoplasia,and 14 cases were cervical cancer.The positive rate of HPV-DNA was significantly higher than that of HPV-E6 protein in cervical precancerous lesions (P ＜ 0.05).The detection rate of cervical cancer was 100％.In comparison of the detection rate of intraepithelial neoplasia and cervical cancer,there were significant differences between the three groups (P ＜ 0.05),and the positive rate of combined detection was higher than that of HPV-DNA and HPVE6 protein.Conclusion With the increasing severity of cervical lesions,the positive detection rate increases gradually.HPV-DNA has higher detection sensitivity,and detection of HPV-E6 protein has high specificity.The combined detection can improve the sensitivity of cervical lesions.