Abstract: Objective To evaluate effect of the comprehensive nursing intervention in prevention of ventilator-associated pneumonia (VAP) in craniocerebral injury patients with mechanical ventilation.Methods A total of 50 mechanical ventilation patients with craniocerebral injury from July to December 2015 were selected as observation group given comprehensive nursing intervention and another 50 mechanical ventilation patients with craniocerebral injury from January to June 2015 were as control group with routine nursing.The situation of expectoration,odor oral cavity,the occurrence of VAP,and prognosis were compared.Results There was significant difference in the amount of expectoration,odor oral cavity,the occurrence of VAP and oral ulcer incidence in two groups (P ＜ 0.05);Mechanical ventilation time,GCS prognosis and ICU stay time showed a significant difference between two groups (P ＜ 0.05).Conclusion Comprehensive nursing intervention can improve expectoration effect of patients with craniocerebral injury,decrease the occurrence of VAP,and improve the prognosis of patients,so it is worth promoting.