PIAS-1基因真核表达质粒的构建与鉴定

The Construction and authentication PIAS-1 gene expression of eukaryotic plasmid

摘要: 目的：构建 PIAS-1 siRNA 干扰载体 Plko．1、PIAS-1。方法设计以 PIAS-1基因为靶标的 siRNA 序列，并克隆人载体 Plko．1，用基因测序进行重组克隆验证。结果PCR 检测证实 siRNA 插入 Plko．1质粒，测序分析证实插入序列正确。结论成功构建 PIAS-1基因的 siRNA 载体，为研究 PIAS-1基因对宫颈癌细胞增殖凋亡的影响提供了稳定转染的骨架载体。

Abstract: Objective To construct interference vector of Plko.1 and PIAS-1.Methods Sequence of siRNA targeted by PIAS-1 was designed and was cloned into the expression vector pLKO. 1.The recombinants were identified by gene sequencing.Results PCR test and gene sequencing a-nalysis showed that the recombinant plasmid was constructed correctly.Conclusion Sequence of siR-NA targeted by PIAS-1 has been constructed successfully,and it provides stable transfection of RNA interference vector for the proliferation and apoptosis of PIAS-1 in cervical cancer.