Abstract: Objective To explore the application effect of detailed nursing on ensuring nursing safety in operation room.Methods 200 patients were divided into control group (50 pa-tients)and observation group(150 patients)according to different nursing methods in operation room.The control group was received routine nursing while the observation group was given de-tailed nursing based on the control group,and nursing quality and satisfaction were compared be-tween the two groups.Results Instrument preparation,nurse coordination skills,equipment management and quality score of disinfection and isolation and safety score of nursing quality in the observation group were higher than that in the control group,the difference was statistical signifi-cant (P <0.05).The difference was not statistical significant in operation time between two groups (P >0.05).Score of image and occupation accomplishment of nursing staff,intraoperative nursing comprehensive score and nursing satisfaction in the observation group were higher than that in the control group,the result revealed that the difference was statistically significant(P <0.05). Conclusion Detailed nursing in operation room can ensure the operation safety and increase pa-tients’satisfaction.