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碱性"彗星"法检测肺癌放射敏感性初步研究

吕纪马, 王绿化, 杨伟志, 张晓华, 王冕荣, 阎洁

吕纪马, 王绿化, 杨伟志, 张晓华, 王冕荣, 阎洁. 碱性"彗星"法检测肺癌放射敏感性初步研究[J]. 实用临床医药杂志, 2005, (9): 63-66. DOI: 10.3969/j.issn.1672-2353.2005.09.015
引用本文: 吕纪马, 王绿化, 杨伟志, 张晓华, 王冕荣, 阎洁. 碱性"彗星"法检测肺癌放射敏感性初步研究[J]. 实用临床医药杂志, 2005, (9): 63-66. DOI: 10.3969/j.issn.1672-2353.2005.09.015
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LV Ji-ma, WANG Lu-hua, YANG Wei-zhi, ZHANG Xiao-hua, WANG Mian-rong, YAN Jie. PRELIMINARY RESULTS OF RADIOSENSITIVITY DETECTED BY ALKALINE COMET ASSAY IN LUNG CANCER PATIENTS[J]. Journal of Clinical Medicine in Practice, 2005, (9): 63-66. DOI: 10.3969/j.issn.1672-2353.2005.09.015
Citation: LV Ji-ma, WANG Lu-hua, YANG Wei-zhi, ZHANG Xiao-hua, WANG Mian-rong, YAN Jie. PRELIMINARY RESULTS OF RADIOSENSITIVITY DETECTED BY ALKALINE COMET ASSAY IN LUNG CANCER PATIENTS[J]. Journal of Clinical Medicine in Practice, 2005, (9): 63-66. DOI: 10.3969/j.issn.1672-2353.2005.09.015
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碱性"彗星"法检测肺癌放射敏感性初步研究

  • 中国医学科学院北京协和医科大学肿瘤医院,北京,100021

详细信息

  • 中图分类号: R734.2

PRELIMINARY RESULTS OF RADIOSENSITIVITY DETECTED BY ALKALINE COMET ASSAY IN LUNG CANCER PATIENTS

摘要

    摘要
  • 摘要: 目的采用碱性彗星法检测肺癌标本的初始DNA单链断裂,探讨碱性彗星法用于预测临床肺癌的放射敏感性的可行性.方法对2002年4月～2003年8月在本院经纤维支气管镜取样并为病理证实的150例肺癌标本采用碱性彗星法检测初始DNA单链断裂,以经本底较正的平均尾力矩(RTM)和平均中位尾力矩(mRTM)为评价指标;采用SPSS 10.0统计软件包以单因素方差分析法比较不同病理类型平均较正尾力矩差异,并分析影响结果的相关因素.结果纤维支气管镜取样,多数样品细胞数较少;样品质量对结果影响较大,35%样品中位RTM≤1.0,无显著性差异,全组平均较正尾力矩在不同病理类型中的分布均未显示出与临床一致的趋势,在细胞数小于100的样品中可见小细胞大于非小细胞的趋势;全组平均中位较正尾力矩尤其在中位RTM＞1.0的样本中,显示与临床放射反应一致分布趋势,但样本小,结果无统计学差异.结论碱性彗星法可初步显示不同病理类型肺癌细胞间的放射敏感性差异,样品质量对结果影响较大,分析方法有待改进完善.
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  • 发布日期:  2005-11-30

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